Screening of Accessions of Adzuki Beans (Vigna angularis (Willd.) Ohwi & Ohashi) Against Root-Knot Nematode, Meloidogyne incognita
2017
Chaudhary, Sonam | Kamaraju, Divya | Phani, Victor | Rav, Umā
Adzuki bean is an important proteinaceous pulse grown mostly in Eastern parts of Asia. Under natural conditions, this crop is attacked by various nematodes including root-knot nematode, Meloidogyne incognita. Apart from being used as an edible pulse, the crop has got large attention in different laboratories for multiplication of Meloidogyne spp. under soil less CYG system as a susceptible host. In the present study, 29 adzuki genotype accessions were screened against M. incognita using CYG germination pouch system. The results revealed that all the accessions showed varying degrees of susceptibility to M. incognita. Based on the infection parameters used for plant susceptibility towards nematode, the genotypes were categorized into three different groups based on the multiplication factor and exhibited variable level of pathogenic success amongst the accessions. Use of CYG growth media provided a good alternative in comparison to soil based microcosms, to get a clean and contamination free synchronized nematode infection. Additionally, keeping of infection track record and avoiding co-contamination by fungal or bacterial pathogens resulted in higher resolution of screening the genotypes. Thus, this soil free approach is independent of season, space saving, stringent and highly useful to prevent any chances of disease escape due to extraneous factors.
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