Enantioseparation of DL‐isocitric acid by a chiral ligand exchange CE with Ni(II)‐D‐quinic acid system
2010
Kodama, Shuji | Taga, Atsushi | Yamamoto, Atsushi | Itō, Yuji | Honda, Yoshitaka | Suzuki, Kentaro | Yamashita, Tomohisa | Kemmei, Tomoko | Aizawa, Sen‐ichi
The ratio of citric acid to D‐isocitric acid can be used to distinguish authentic and adulterated fruit juices. To separate DL‐isocitric acid enantiomers, we used ligand exchange CE. D‐Quinic acid was used as a chiral selector ligand and Mn(II), Fe(III), Co(II), Ni(II), Cu(II), and Zn(II) ions were used as the central ions of the chiral selector in the BGE. DL‐Isocitric acid was found to be enantioseparated with the above metal ions except for Mn(II) ion. The optimum running conditions for the analysis of D‐ and L‐isocitric acids along with citric acid, an isomer of isocitric acid, were found to be a BGE (pH 5.0) containing 30% ACN, 20 mM acetic acid, 20 mM NiSO₄, and 80 mM D‐quinic acid. Under these conditions, DL‐isocitric and citric acids in fruit juices were analyzed successfully.
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