Stabilization of semi-solid-state starch by branching enzyme-assisted chain-transfer catalysis at extreme substrate concentration
2013
Jensen, Susanne L. | Larsen, Flemming H. | Bandsholm, Ole | Blennow, Andreas
In this study a branching enzyme (BE, α-1,4→α-1,6 glycosyltransferase) was used to modify starch granules of different structures at high temperature and at extreme starch dry matter content (30–40%, w/v) to restrict temperature-induced swelling. As opposed to diluted systems, such conditions led to stabilization of the granular structure for low-phosphate starch types at the highest BE activity. Bright field/polarized light and scanning electron microscopy confirmed maintenance of granular structure. The product compared to the control had significantly increased degree of branching as identified by shorter branch-length of the α-1,4 chains assessed by chromatography and larger proportion of α-1,6 links to α-1,4 links as assessed by 1H nuclear magnetic resonance spectroscopy. Size exclusion chromatography demonstrated the presence of uniform molecules with smaller size. Further the polysaccharide product was 40% more soluble at 25°C than the corresponding heat treated control. Both of these observations were supported by 13C solid-state MAS NMR. Hence, significant chain transfer took place in the semi-solid state starch permitting conservation of the main granular organization in the final product. A hypothetic model is presented to account for the observed phenomenon.
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