Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide
2012
Liu, Bing | Garnett, James A. | Lee, Wei-chao | Lin, Jing | Salgado, Paula | Taylor, Jonathan | Xu, Yingqi | Lambert, Sebastian | Cota, Ernesto | Matthews, Steve
The use of heavy water (D₂O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D₂O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.
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