In vitro tuberization of Xanthosoma sagittifolium L. Schott: effects of phytohormones, sucrose, nitrogen and photoperiod
2003
Omokolo, N.D. | Boudjeko, T. | Takadong, J.JTsafack
Shoot tips of cocoyam (Xanthosoma sagittifolium L. Schott) cv. White were induced to develop multiple shoots in a Murashige and Skoog mineral salt medium supplemented with vitamins, 3% sucrose, 0.3% agar and 5 μM benzylaminopurine (BAP). Shoots were separated and used as explants for the production of microtubers. Tuberization was observed in media containing BAP. This process was accompanied by budding and/or rhizogenesis. The highest % of tuberization (83%) was obtained with 30 μM BAP and 80 g/l sucrose under SD-dark regime. The number of microtubers per responding explant produced under LD was more abundant (up to 12 microtubers per explant) but had the lowest fresh weight (0.2 g per microtuber). The highest fresh weight per microtuber (0.9 g) was obtained in LD-dark regime with 80 g/l sucrose and 30 μM BAP. Tuberization was also induced in growth regulator-free media by varying the NO3−:NH4+ ratio under SD-dark. The highest number of microtubers (about 3.5) per shoot and the highest fresh weight of microtubers per plantlet (about 0.5 g) were obtained with 1:1 and 2:1 ratios.
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