Understanding the phytotoxic impact of Al3+, nano-size, and bulk Al2O3 on growth and physiology of maize (Zea mays L.) in aqueous and soil media
2022
Ahmed, Bilal | Rizvi, Asfa | Syed, Asad | Rajput, Vishnu D. | Elgorban, Abdallah M. | Al-Rejaie, Salim S. | Minkina, Tatiana | Khan, Mohammad Saghir | Lee, Jintae
The release and accumulation of metal-oxide nanoparticles in soils have threatened terrestrial plants. However, limited knowledge is available on the accumulation of nano-Al₂O₃ (22 nm), bulk-Al₂O₃ (167 nm), and Al³⁺ by maize plants and the subsequent impact on its physiology and growth in agar (0.7% w/v), hydroponic (1X), and soil. Maize plants were cultivated with 0.05–2 mg g⁻¹ or ml⁻¹ of three Al types and their biological attributes, oxidative status, Al bioaccumulation, and translocation were measured. The ICP-MS results revealed a dose-dependent increase (P ≤ 0.05 or ≤0.01) in Al content in maize tissues following nano-Al₂O₃ and Al³⁺ exposure, however, plants exposed to bulk-Al₂O₃ showed no significant uptake of Al. Atomic mapping by EDX during SEM analysis and TEM revealed varied distributions of nano-Al₂O₃ from roots to aerial parts and intracellular transportation. Al deposition in tissues followed the order: Al³⁺ > nano-Al₂O₃ > bulk-Al₂O₃ and therefore, a similar trend of toxicity was observed for seed germination, the emergence of plant organs, length, biomass accumulation, total chlorophyll, phosphorus content, and total soluble protein. Oxidative stress was profoundly induced dose-dependently and was highest at 2 mg ml⁻¹ or g⁻¹ of Al³⁺ and nano-Al₂O₃ when superoxide radical formation, proline induction, activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (GPX), and glutathione reductase (GR) and membrane lipid peroxidation were measured. Aluminum toxicity was found higher in hydroponically grown maize compared to soil-grown maize. Forty days exposure in soil showed greater inhibition of maize growth compared to 20 days exposure. This study is significant in understanding the maize response to different Al types in soil and soil-free media.
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