Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)
2008
Pérez-Pérez, José M. | Serralbo, Olivier | Vanstraelen, Marleen | González, Cristina | Criqui, Marie-Claire | Genschik, Pascal | Kondorosi, Eva | Scheres, Ben
To investigate the specialization of the two Arabidopsis CDC27 subunits in the anaphase-promoting complex (APC/C), we analyzed novel alleles of HBT/CDC27B and CDC27A, and characterized the expression of complementing HOBBIT (HBT) protein fusions in plant meristems and during the cell cycle. In contrast to other APC/C mutants, which are gametophytic lethal, phenotypes of weak and null hbt alleles indicate a primary role in the control of post-embryonic cell division and cell elongation, whereas cdc27a nulls are phenotypically indistinguishable from the wild type. However, cdc27a hbt double-mutant gametes are non-viable, indicating a redundant requirement for both CDC27 subunits during gametogenesis. Yeast-two-hybrid and pulldown studies with APC/C components suggest that the two Arabidopsis CDC27 subunits participate in several complexes that are differentially required during plant development. Loss-of-function analysis, as well as cyclin B reporter protein accumulation, indicates a conserved role for the plant APC/C in controlling mitotic progression and cell differentiation during the entire life cycle.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил National Agricultural Library