Lactulose and mannitol as probe markers for in vivo assessment of passive intestinal permeability in healthy cats

Papasouliotis, K.; Gruffydd-Jones, T.J.; Sparkes, A.H.; Cripps, P.J.; Millard, W.G.

Lactulose and mannitol as probe markers for in vivo assessment of passive intestinal permeability in healthy cats

1993

Papasouliotis, K. | Gruffydd-Jones, T.J. | Sparkes, A.H. | Cripps, P.J. | Millard, W.G.

Intestinal permeability was assessed in 12 healthy cats by use of a differential sugar absorption test. A 50-ml isotonic aqueous solution containing a combination of 1.8 g of the disaccharide lactulose and 1.7 g of the monosaccharide mannitol was administered to cats via nasogastric tube. Urine was collected after 6 hours, and all urine samples were analyzed the same day, using a gas-liquid chromatographic technique (GLC) and an enzymatic assay (ENZ). Median urinary recovery of lactulose was 0.27 and 0.54% determined by GLC and ENZ, respectively. Differences between these groups were statistically significant (P = 0.023), and correlation between assays was high (r = 0.94, P < 0.01). Median urinary recovery of mannitol was 1.93 and 2.09% for GLC and ENZ, respectively. There were no statistically significant differences between these groups and the correlation between assays was high (r = 0.85, P < 0.01). The median lactulose-to-mannitol ratio was 0.29, using GLC, and was 0.52, using ENZ. Correlation of these ratios was again high (r = 0.93, P < 0.01).

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