Nitratireductor mangrovi sp. nov., a Nitrate-Reducing Bacterium Isolated from Mangrove Soil
2020
Ye, Yanghui | Yan, Cen | Nie, Yanfang | Zhang, Jianwei | Zhao, Zhe | Zhang, Ran | Xiao, Chuan | Wu, Min
A Gram-stain-negative, non-motile and short-rod-shaped bacterium, designated as strain SY7ᵀ, was isolated from rhizosphere soil of the mangrove Kandelia obovata of Fugong village, in Zhangzhou, China. The isolate grew at 10–45 °C (optimum 30 °C), pH 6.0–10.0 (optimum pH 7.0) and 0–8% NaCl (optimum 3%, w/v). The 16S rRNA gene sequence and phylogenetic analysis revealed that strain SY7ᵀ located within the radiation of genus Nitratireductor and showed the highest sequence similarity of 97.23% to Nitratireductor pacificus MCCC 1A01024ᵀ. The DNA G+C content was 64.9%. In silico DNA–DNA hybridization and average nucleotide identity values between strain SY7ᵀ with reference strains of N. pacificus MCCC 1A01024ᵀ, N. basaltis KCTC 22119ᵀ and N. aquibiodomus DSM 15645ᵀ were 16.7%, 14.3%, 14.7% and 75.2%, 72.6%, 73.5%, respectively. The major isoprenoid quinone was Q-10. The dominant fatty acids were 11-methyl C₁₈:₁ω7c, iso-C₁₇:₀, C₁₉:₀ω8c cyclo and summed feature 8 (C₁₈:₁ω6c/C₁₈:₁ω7c), a profile that almost matched the other members of the genus Nitratireductor. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol. On the basis of the phenotypic, phylogenetic and chemotaxonomic analysis, strain SY7ᵀ represents a novel species of the genus Nitratireductor, for which the name Nitratireductor mangrovi sp. nov., is proposed. The type strain is SY7ᵀ (= KCTC 72110ᵀ = MCCC 1K03723ᵀ).
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