Present status of research on the etiology of the full blossom disease in red and white currants in the Czech Republic
2004
Špak, J. | Přibylová, J. | Kubelková, D. | Špaková, V. | Navrátil, M. | Válová, P. | Fialová, R.
The presence of phytoplasmas in the shrubs of red and white currants exhibiting full blossom symptoms was determined by nested PCR with primers amplifying phytoplasma 16S rRNA gene, sequencing and by electron microscopy. In total, 6 of 10 analysed symptomatic bushes gave positive PCR reaction. Based on the results of sequencing the phytoplasma isolates were identified as belonging to the apple proliferation group and aster yellows group. The presence of phytoplasma-like bodies in the phloem of two red currant bushes infected with aster yellows and apple proliferation phytoplasmas was confirmed by electron microscopy. All 10 analysed bushes were to be found infected with the Blackcurrant reversion virus. In graft experiments, the disease was transmitted from infected bushes to red currant 'Rondom', 'Jonkheer van Tets' and 'Vitan', white currant 'Blanka' and black currant 'Viola'. Red and white currants showed symptoms of full blossom, whereas 'Viola' symptoms of the severe form of reversion.
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