Isolation, identification, and crude oil degradation characteristics of a high-temperature, hydrocarbon-degrading strain

Liu, Boqun; Ju, Meiting; Liu, Jinpeng; Wu, Wentao; Li, Xiaojing

Isolation, identification, and crude oil degradation characteristics of a high-temperature, hydrocarbon-degrading strain

2016

Liu, Boqun | Ju, Meiting | Liu, Jinpeng | Wu, Wentao | Li, Xiaojing

In this work, a hydrocarbon-degrading bacterium Y-1 isolated from petroleum contaminated soil in the Dagang Oilfield was investigated for its potential effect in biodegradation of crude oil. According to the analysis of 16S rRNA sequences, strain Y-1 was identified as Bacillus licheniformis. The growth parameters such as pH, temperature, and salinity were optimised and 60.2% degradation of crude oil removal was observed in 5days. The strain Y-1 showed strong tolerance to high salinity, alkalinity, and temperature. Emplastic produced by strain Y-1 at high temperatures could be applied as biosurfactant. Gas chromatography analysis demonstrated that the strain Y-1 efficiently degraded different alkanes from crude oil, and the emplastic produced by strain Y-1 promoted the degradation rates of long-chain alkanes when the temperature increased to 55°C. Therefore, strain Y-1 would play an important role in the area of crude oil contaminant bioremediation even in some extreme conditions.

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