Simultaneous determination of 2-naphthol and 1-hydroxy pyrene in urine by gas chromatography–mass spectrometry
2011
Shin, Ho-Sang | Lim, Hyun-Hee
A gas chromatography–mass spectrometric (GC–MS) method was developed for the determination of 2-naphthol (2-NAP) and 1-hydroxypyrene (1-HOP) in human urine. Extraction from urine after the enzyme hydrolysis with β-glucuronidase/arylsulfatase was achieved with a liquid extraction using 5mL of pentane. After addition of 50μL of N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBDMSTFA) to prevent the loss of 2-NAP during drying, the extract was completely dried and derivatized with MTBDMSTFA for 30min at 60°C. The accuracies were in the range of 96–109% at a concentration of 0.5, 10 and 25μg/L and their precisions were less than 15%. Method detection limits of 2-NAP and 1-HOP were 0.07 and 0.01μg/L, respectively. This method was used to analyze twenty urine samples, and they were found in the concentration range <0.07–13.7μg/L (2-NAP) and <0.01–0.88μg/L (1-HOP). The concentrations of 2-NAP and 1-HOP were well correlated to those of naphthalene and pyrene in blood, respectively.
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