Effect of various cytokinins and auxins on in vitro regeneration of plantlets from isolated bud clumps of Silk Banana var. Nanjanagud Rasabale (Musa AAB)
2016
Waman, Ajit Arun | Bohra, Pooja | Sathyanarayana, B. N.
Separated bud clumps of Silk Banana containing single and double buds were transferred on media containing six different cytokinins at three different levels and the proliferation capacity was determined. Proliferation, though occurred in single bud explants, the rate of multiplication was lower when compared with double bud explants, irrespective of the type of cytokinin used. Maximum shoot proliferation was observed in explants cultured on media supplemented with thidiazuron and 2-isopentenyl adenine (2-iP), and was significantly superior when combined with kinetin and BAP. Meta-topolin though reported to be highly efficient in improving multiplication in other banana varieties, no appreciable response was observed during present investigation. Use of NAA (2 mg l⁻¹) was found to promote rooting process with superior root induction parameters and survival.
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