Hydrogen peroxide biosensor based on microperoxidase-11 immobilized on flexible MWCNTs-BC nanocomposite film
2015
Zhang, Bingyan | Zhou, Jianhai | Li, Shaohui | Zhang, Xiaofan | Huang, Dekang | He, Yahui | Wang, Mingkui | Yang, Guang | Shen, Yan
In the present work, we report on an experimental study of flexible nanocomposite film for electrochemical detection of hydrogen peroxide (H2O2) based on bacterial cellulose (BC) and multi-walled carbon nanotubes (MWCNTs) in combination with microperoxidase-11 (MP-11). MWCNTs are used to functionalize BC and provide a flexible conductive film. On the other hand, BC can improve MWCNTs׳ biocompatibility. The investigation shows that MP-11 immobilized on the flexible film of MWCNTs–BC can easily present a pair of well-defined and quasi-reversible redox peaks, revealing a direct electrochemistry of MP-11 on the nanocomposite film. The apparent heterogeneous electron-transfer rate constant ks is estimated to be 11.5s⁻¹. The resulting flexible electrode presents appreciated catalytic properties for electrochemical detection of H2O2, comparing to traditional electrodes (such as gold, glassy carbon electrode) modified with MP-11. The proposed biosensor exhibits a low detection limit of 0.1µM (at a signal-to-noise ratio of 3) with a linear range of 0.1–257.6µM, and acquires a satisfactory stability.
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