A polyphenol-rich plant extract prevents hypercholesterolemia and modulates gut microbiota in western diet-fed mice
2024
Langhi, Cédric | Vallier, Marie | Bron, Auriane | Otero, Yolanda, F | Maura, Maheva | Le Joubioux, Florian | Blomberg, Niek | Giera, Martin | Guigas, Bruno | Maugard, Thierry | Chassaing, Benoit | Peltier, Sébastien | Blanquet-Diot, Stéphanie | Bard, Jean-Marie | Sirvent, Pascal | Valbiotis R & D [Riom] ; Valbiotis SAS ; Partenaires INRAE-Partenaires INRAE | Microbiologie Environnement Digestif Santé (MEDIS) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA) | Valbiotis R & D [La Rochelle] ; Valbiotis SAS ; Partenaires INRAE-Partenaires INRAE | Leiden University Medical Center (LUMC) ; Universiteit Leiden = Leiden University | LIttoral ENvironnement et Sociétés (LIENSs) ; Institut national des sciences de l'Univers (INSU - CNRS)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) | Institut Cochin (IC UM3 (UMR 8104 / U1016)) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) | Valbiotis R&D [Perigny] ; Valbiotis SAS ; Partenaires INRAE-Partenaires INRAE | Institut Des Substances et Organismes de la Mer - UR 2160 (ISOMER) ; Nantes Université - UFR des Sciences Pharmaceutiques et Biologiques (Nantes Univ - UFR Pharmacie) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST) ; Nantes Université - pôle Sciences et technologie ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie ; Nantes Université (Nantes Univ) | European Project: 2015RPC-BAFE-114
International audience
Показать больше [+] Меньше [-]Английский. Introduction Totum-070 is a combination of five plant extracts enriched in polyphenols to target hypercholesterolemia, one of the main risk factors for cardiovascular diseases. The aim of this study was to investigate the effects of Totum-070 on cholesterol levels in an animal model of diet-induced hypercholesterolemia. Methods C57BL/6JOlaHsd male mice were fed a Western diet and received Totum-070, or not, by daily gavage (1g/kg and 3g/kg body weight) for 6 weeks. Results The Western diet induced obesity, fat accumulation, hepatic steatosis and increased plasma cholesterol compared with the control group. All these metabolic perturbations were alleviated by Totum-070 supplementation in a dose-dependent manner. Lipid excretion in feces was higher in mice supplemented with Totum-070, suggesting inhibition of intestinal lipid absorption. Totum-070 also increased the fecal concentration of short chain fatty acids, demonstrating a direct effect on intestinal microbiota. Discussion The characterization of fecal microbiota by 16S amplicon sequencing showed that Totum-070 supplementation modulated the dysbiosis associated with metabolic disorders. Specifically, Totum-070 increased the relative abundance of Muribaculum (a beneficial bacterium) and reduced that of Lactococcus (a genus positively correlated with increased plasma cholesterol level). Together, these findings indicate that the cholesterol-lowering effect of Totum-070 bioactive molecules could be mediated through multiple actions on the intestine and gut microbiota.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Institut national de la recherche agronomique