Cryoprotectant effects of egg yolk on Indian red jungle fowl (Gallus gallus murghi) sperm
Rakha, B. A. | Ansari, M. S. | Akhter, S. | Santiago-Moreno, J. | Blesbois, Elisabeth | Department of Wildlife Management ; Pir Mehr Ali Shah Arid Agriculture University = PMAS-Arid Agriculture University Rawalpindi (AAUR) | University of Lahore Sargodha ; Partenaires INRAE | Department of Zoology ; University of Gujrat | Department of Animal Reproduction ; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA) | Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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Показать больше [+] Меньше [-]Английский. Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 +/- 2.5%), plasma membrane integrity (663 +/- 2.4%), viability (58.8 +/- 1.3%) and acrosomal integrity (60.0.8 +/- 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 +/- 0.7, 21.6 +/- 1.2), percent fertility (55.9 +/- 4.4, 46.5 +/- 2.2), no. of hatched chicks (23.6 +/- 1.0,17.2 +/- 1.0), percent hatch (49.5 +/- 3.2, 37.1 +/- 2.5%) and hatchability of the fertile eggs (89.4 +/- 2.2, 79.7 +/- 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm.
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