The selenium-binding protein of Theobroma cacao: A thermostable protein involved in the witches’ broom disease resistance
2019
Martins Alves, Akyla Maria | Pereira Menezes, Sara | Matos Lima, Eline | Peres Gramacho, Karina | Silva Andrade, Bruno | Macêdo Ferreira, Monaliza | Pirovani, Carlos Priminho | Micheli, Fabienne | Universidade Estadual De Santa Cruz [Brazil] (UESC) | Cocoa Research Center CEPEC-CEPLAC | Universidade Estadual do Sudoeste da Bahia (UESB) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB) | Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) | Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) | Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)DTE0038/2013
International audience
Показать больше [+] Меньше [-]Английский. The selenium-binding proteins are known to be inducers of apoptosis in human and animals, and have been studied as target for the treatment of various types of cancer. In plants, SBP expression has been related to abiotic and biotic stress resistance. The SBP from Theobroma cacao (TcSBP) was first identified from a cocoa-Moniliophthora perniciosa cDNA library. The present study provides details on the TcSBP gene and protein structure. Multiple alignments revealed conserved domains between SBP from plants, human and archea. Homology modeling and molecular docking were performed and showed that the TcSBP has affinity to selenite in the active CSSC site. This result was confirmed by circular dichroism of the recombinant TcSBP, which also presented thermostable behavior. RT-qPCR analysis showed that TcSBP was differentially expressed in resistant vs susceptible cacao varieties inoculated by M. perniciosa and its expression was probably due to hormone induction via cis-regulating elements present in its promotor. The presence of the CSSC domain suggested that TcSBP acted by altering oxidation/reduction of proteins during H2O2 production and programmed cell death in the final stages of the witches' broom disease. To our knowledge, this is the first in silk and in vitro analysis of the SBP from cacao.
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