Scale-up production of vitamin loaded heteroprotein coacervates and their protective property
Chapeau, Anne Laure | Hamon, Pascaline | Rousseau, Florence | Croguennec, Thomas | Poncelet, Denis | Bouhallab, Said | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA) ; Institut Universitaire de Technologie - Nantes (IUT Nantes) ; Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST) ; Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire) ; Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN) ; Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique) ; Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon) ; Université de Nantes (UN) | Regional councils of Brittany (grant n 13008651) and Pays de la Loire (grant n 2014-07081) and INRA for the financial support of this work through the interregional project PROFIL, supported by BBA industrial association and managed by the “P^ole Agronomique Ouest”.
The production of biocarriers for the protection and controlled delivery of bioactives is of great interest todevelop functional foods. The ability of two whey proteins (WP), Beta-Lactoglobulin (BLG) and Lactoferrin(LF), to efficiently entrap vitamin B9 by complex coacervation has been reported at laboratoryscale. In the present work, we report on the scaling-up production of B9 loaded BLG-LF coacervates (B9-WP coacervates). Complex coacervation was performed at bench scale, using commercial protein solutions.Under optimized conditions, B9-WP coacervates were produced by static mixing at a flow rate of300 mL/min. Bench scale efficiency similar to that found for laboratory scale was reached with a coacervationyield of 65% and the B9 entrapment of 98%, demonstrating an efficient scaling-up. B9-WP coacervatesshowed good protection property for B9 during storage treatments, confirming the efficiency ofthis type of biocarrier for the development of natural functional foods.
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