Evaluation of the effect of chitosan and collagen extracted from aquatic(shrimp, Rutilus kutum and Scomberomorus guttatus) on wound healing

his study has been conducted to extract collagen from the skin of Rutilus kutum from of the Caspian Sea and Scomberomorus guttatus which is one of the tuna species of the Persian Gulf. Acid-soluble collagens (ASC) from the skin of Scomberomorus guttatus and Rutilus kutum were extracted and characterized by Pico.Tag, SDS-PAGE, FTIR and UV spectrophotometry. Then The collagenchitosan gel extracted from aquatic (shrimp, Rutilus kutum and Scomberomorus guttatus fish) was used to evaluation II grade burn wound treatment. Burn treatment was performed in two steps: 1. First step; Determine the most effective collagen-chitosan gel ratio (collagen / chitosan ratio) with three different ratios (1: 3, 1: 1, 3: 1 Rutilus kutum fish collagen-chitosan( RKC0.75, RKC0.5 and RKC0.25) and Scomberomorus guttatus fish (SGC0.75, SGC0.5 and SGC0.25). The second step was to compare the purpose group (collagen-chitosan gel) with the control and control groups in this The rats were divided into 5 groups. The first group (N = 8): 1% collagen gel, the second group : 1% collagen-chitosan gel, the third group: 1% chitosan gel, 4th group : silver sulfadiazine, Group 5: withoud drug. Burn wound healing was evaluated by two methods of measuring wound area and histopathology. Based on the data from the SDS-PAGE and the composition of amino acid, both collagens were classified as type of collagen I. Collagens consisted of two different types of α chains, including α1 and α2, and β component. Also, FTIR analysis revealed the presence of helical arrangements of collagens. Moreover, UV spectrophotometry exhibited that this collagen had an absorbance at 220 nm. According to the basis of dry weight, the extract of collagen in Rutilus kutum and Scomberomorus guttatus ASC were calculated 15.6 % and 14.5 %. Amino acid analysis revealed a much higher glaycin content in the both collagens extracted which accounted to one third of the total amino acids. Besides, proline as an unique amino acid in Rutilus kutum and Scomberomorus guttatus ASC had the specific amount of 89.6 and 86.8 residues per 706.1 residues respectively. The results of the study to determine the most effective ratio of collagen-chitosan in Rutilus kutum based on wound area measurement showed that the group treated with collagen-chitosan gel with a ratio of 1: 1 (RKC0.5) had the greatest effect on burn wound healing and the group Treatment with RKC0.25 had less effect on burn wound healing than the other two groups. The results of the investigation of determining the most effective collagenchitosan ratio in Scomberomorus guttatus fish based on wound area measurement showed that the treated group with collagen-chitosan gel with a ratio of 1: 3 (SGC0.75) on days 5, 10, 15 and 20 And 25 burns had the least wound area than the other two groups, so it had the greatest effect on burn wound healing and the treatment group with SGC0.25 had less effect on burn wound healing than the other two groups, also the Histopathologic results showed that there was no a significant difference between the groups (SGC0.75 and SGC0.5 and SGC0.25) on 15 and 30 days. (P> 0.05). The results of the comparison purpose group (collagen-chitosan gel) with the control group and the control group in both groups Rutilus kutum and Scomberomorus guttatus fish showed that on the 10th, 15th, 20th and 25th day of burn, the treated group with collagen- Chitosan had a significant difference with the sulfadiazine treated group and the non-drug group (p <0.005). The results also showed that the treated group with collagen-chitosan gel was completely improved on day 25 and the treated group with silver sulfadiazine had the highest wound area among the groups. The results of histopathologic study showed that there was a significant difference between the groups in the 15th and 30th burns (p <0.05), and the treated groups with collagen gel, chitosan gel and collagen-chitosan gel had higher scores and better results than The two groups of silver sulfadiazine and the group did not burn at day 15 and 30 without using the drug. collagen-chitosan gel can be used as a good candid for second burn healing skin.

PhD