Investigating the correlation between sperm quality parameters and the amount of contribution by Caspian brown trout male breeders to F1 progeny in mixed milt fertilization
2014
Sourinejad, Iman | Kalbassi, Mohammad Reza
Artificial breeding and subsequent release of the Caspian brown trout juveniles (Salmo trutta caspius Kessler, from Salmonidae) has been adopted by Iranian Fisheries Organization as a result of fishing pressure on its stocks. Unbalanced contribution of male breeders to F1 progeny is expected with present mixed milt fertilization of gametes. Non-parametric Spearman's correlation coefficient was used to investigate the relationship between some sperm quality parameters and the contribution of Caspian brown trout male breeders at three trials. In the first trial, fertilization was performed using ova and sperm of four male and four female breeders according to routine hatchery protocol. In the second and third trials, ova and sperm of respectively four and two female and four and six male breeders with equal number of ova and the same volume of milt were fertilized. Parentage assignment of yolk sac absorbed F1 alevins was performed in FAP program using three polymorphic microsatellite loci (Str58, Str73 and Str591). More than 94% of mixed milt produced progeny were assigned to their parents in trials. Sperm motility duration was positively correlated with the number of sired progeny by each male in trials. The correlation was negative between sperm concentration and spermatocrit with amount of contribution by male breeders to F1 progeny. The results reveal the effect of sperm motility duration on the ability of alevin production by Caspian brown trout male breeders at sperm competition condition in mixed milt fertilization of gametes.
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