Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light
Bizet, François | Dupuy, Lionel | Bengough, Anthony, Glyn | Peaucelle, Alexis | Hummel, Irène | Bogeat-Triboulot, Marie-Béatrice | Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | The James Hutton Institute | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech
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Показать больше [+] Меньше [-]Английский. Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.
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