TRANSPARENT TESTA 16 and 15 act through different mechanisms to control proanthocyanidin accumulation in Arabidopsis testa
2017
Xu, Wenjia | Bobet, Sophie | Le Gourrierec, José | Grain, Damaris | de Vos, Délphine | Berger, Adeline | Salsac, Fabien | Kelemen, Zsolt | Boucherez, Jossia | Rolland, Aurélia | Mouille, Gregory | Routaboul, Jean-Marc | Lepiniec, Loic | Dubos, Christian | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Génomique et Biotechnologie des Fruits (GBF) ; Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse (ENSAT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)
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Показать больше [+] Меньше [-]Английский. Flavonoids are secondary metabolites that fulfil a multitude of functions during the plant life cycle. In Arabidopsisproanthocyanidins (PAs) are flavonoids that specifically accumulate in the innermost integuments of the seedtesta (i.e. endothelium), as well as in the chalaza and micropyle areas, and play a vital role in protecting the embryoagainst various biotic and abiotic stresses. PAs accumulation in the endothelium requires the activity of the MADSbox transcription factor TRANSPARENT TESTA (TT) 16 (ARABIDOPSIS B-SISTER/AGAMOUS-LIKE 32) and the UDPglycosyltransferaseTT15 (UGT80B1). Interestingly tt16 and tt15 mutants display a very similar flavonoid profiles andpatterns of PA accumulation. By using a combination of genetic, molecular, biochemical, and histochemical methods,we showed that both TT16 and TT15 act upstream the PA biosynthetic pathway, but through two distinct geneticroutes. We also demonstrated that the activity of TT16 in regulating cell fate determination and PA accumulation in theendothelium is required in the chalaza prior to the globular stage of embryo development. Finally this study providesnew insight showing that TT16 and TT15 functions extend beyond PA biosynthesis in the inner integuments of theArabidopsis seed coat.
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