Hierarchical shift of the Aedes albopictus microbiota caused by antimicrobiota vaccine increases fecundity and egg-hatching rate in female mosquitoes
2023
Mateos-Hernández, Lourdes | Maitre, Apolline | Abuin-Denis, Lianet | Obregon, Dasiel | Martin, Edwige | Luis, Patricia | Maye, Jennifer | Wu-Chuang, Alejandra | Valiente Moro, Claire | Cabezas-Cruz, Alejandro | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Systèmes d'Elevage Méditerranéens et Tropicaux - Laboratoire de Recherche sur le Développement de l'Elevage (SELMET-LRDE) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Università di Corsica Pasquale Paoli [Université de Corse Pascal Paoli] | Center for Genetic Engineering and Biotechnology [Cuba] (CIGB) | University of Guelph [Guelf, Ontario, Canada] | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | SEPPIC ; SEPPIC | BIPAR was funded by the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” (grant number ANR-10-LABX-62-IBEID). A.W.-C. was supported by Programa Nacional de Becas de Postgrado en el Exterior “Don Carlos Antonio López” (grant number 205/2018). A.M. is supported by the “Collectivité de Corse” grant: “Formations superieures” (SGCE-RAPPORT number 0300). | ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
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Показать больше [+] Меньше [-]Английский. Recent studies show that mosquito–microbiota interactions affects vector competence and fitness. We investigated if host antibodies modifying microbiota impact mosquito physiology. We focused on three prevalent bacteria (Acinetobacter, Pantoea, and Chryseobacterium), originally isolated from the Asian tiger mosquito Aedes albopictus. Our goal was to assess the impact of host antibodies on mosquito microbiota and life traits. Female mosquitoes were fed with blood from rabbits immunized with each bacterium or a mock vaccine. We compared various factors, including feeding behavior, survival rates, and reproductive success of the mosquitoes. Interestingly, mosquitoes fed with blood from a Chryseobacterium-immunized rabbit showed a significant increase in fecundity and egg-hatching rate. This outcome correlated with a decrease in the abundance of Chryseobacterium within the mosquito microbiota. While no significant changes were observed in the alpha and beta diversity indexes between the groups, our network analyses revealed an important finding. The antimicrobiota vaccines had a considerable impact on the bacterial community assembly. They reduced network robustness, and altered the hierarchical organization of nodes in the networks. Our findings provide the basis for the rational design of antimicrobiota vaccines to reduce mosquito fitness and potentially induce infection-refractory states in the microbiota to block pathogen transmission.
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