Effects of dihydropyrimidinase-related protein 2 on anxiety level in rats
2024
Guliyeva, Shahla | Humbatov, Sovet | Huseynzadeh, Sima | Mekhtiev, Arif
The article concerns studies on molecular mechanisms of anxiety. The studies were carried out on Wistar male rats, through the application of an open field test and an elevated plus-maze model. On the audiogenic stress model, stress-resistant rats were chosen for the following studies. The animals were divided into 3 groups: 1) intact group, 2) control group – inactive dihydropyrimidinase related protein 2 (DRP2), and 3) experimental group – DRP2. Preparations (1 mg/mL, 10 µL) were administered into the brain lateral ventricle of etherized rats 24 h prior to behavioral studies. The results of the studies carried out on the open field test revealed a decline in the number of rearings (p<0.01), the total duration of grooming (p<0.001), the number of crossed squares (p<0.01), and an upregulation of the total duration of freezing in the experimental group (p<0.01) relative to the control animals. The results obtained in the elevated plus-maze model showed a sharp decline in the number of rearings (p<0.001), total duration of grooming (p<0.001), a number of crossed squares (p<0.001), a three-fold increase of total duration of freezing time (p<0.001) in the closed branch and a prominent, more than four-time decrease in the number of hangings in the experimental animals (p<0.001) relative to the control ones. A general conclusion concerning the anxiogenic effects of intra-cerebral administration of DRP2 on animal behavior is put forward.
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