What Do Pneumocystis Organisms Tell Us about the Phylogeography of Their Hosts? The Case of the Woodmouse Apodemus sylvaticus in Continental Europe and Western Mediterranean Islands
Demanche, Christine | Deville, Manjula | Michaux, Johan | Barriel, Véronique | Pinçon, Claire | Aliouat-Denis, Cécile Marie | Pottier, Muriel | Noël, Christophe | Viscogliosi, Eric | Aliouat, El Moukhtar | Dei-Cas, Eduardo | Morand, Serge | Guillot, Jacques | Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) ; Institut Pasteur de Lille ; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [CHU Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire de parasitologie EA4547 ; Centre Hospitalier Régional Universitaire [CHU Lille] (CHRU Lille) | École nationale vétérinaire d'Alfort (ENVA) | Centre de Biologie pour la Gestion des Populations (UMR CBGP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [Occitanie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Institut de Botanique [Liège] ; Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège) | Centre de recherche sur la Paléobiodiversité et les Paléoenvironnements (CR2P) ; Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) | Evaluation des technologies de santé et des pratiques médicales - ULR 2694 (METRICS) ; Université de Lille-Centre Hospitalier Régional Universitaire [CHU Lille] (CHRU Lille) | Geneius Laboratories Ltd, INEX business Centre, Newcastle | Parasitologie-Mycologie EA4547 ; Centre Hospitalier Régional Universitaire [CHU Lille] (CHRU Lille) | Institut des Sciences de l'Evolution de Montpellier (UMR ISEM) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS) | French Ministry of Research (Lille Nord de France University) [EA4547]; French Ministry of Research (Lille Pasteur Institute); French Institute of Biodiversity (IFB)
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Показать больше [+] Меньше [-]Английский. Pneumocystis fungi represent a highly diversified biological group with numerous species, which display a strong host-specificity suggesting a long co-speciation process. In the present study, the presence and genetic diversity of Pneumocystis organisms was investigated in 203 lung samples from woodmice (Apodemus sylvaticus) collected on western continental Europe and Mediterranean islands. The presence of Pneumocystis DNA was assessed by nested PCR at both large and small mitochondrial subunit (mtLSU and mtSSU) rRNA loci. Direct sequencing of nested PCR products demonstrated a very high variability among woodmouse-derived Pneumocystis organisms with a total number of 30 distinct combined mtLSU and mtSSU sequence types. However, the genetic divergence among these sequence types was very low (up to 3.87%) and the presence of several Pneumocystis species within Apodemus sylvaticus was considered unlikely. The analysis of the genetic structure of woodmouse-derived Pneumocystis revealed two distinct groups. The first one comprised Pneumocystis from woodmice collected in continental Spain, France and Balearic islands. The second one included Pneumocystis from woodmice collected in continental Italy, Corsica and Sicily. These two genetic groups were in accordance with the two lineages currently described within the host species Apodemus sylvaticus. Pneumocystis organisms are emerging as powerful tools for phylogeographic studies in mammals.
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