Metagenomic data of free cyanide and thiocyanate degrading bacterial communities
Mekuto, Lukhanyo | Ntwampe, Seteno Karabo Obed | Mudumbi, John Baptist Nzukizi | Akinpelu, Enoch Akinbiyi | Mewa-Ngongang, Maxwell
The datapresented in this article contains the bacterial community structure of the free cyanide(CN-) and thiocyanate(SCN-) degrading organisms that were isolated from electroplating was- tewater and synthetic SCN- containing wastewater.PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the meta- community DNA extraction procedure.The PCR amplicons were processed using the illumina reaction kits as per manufacturer's instruction and sequenced using the illumina MiSeq-2000, using the MiSeq V3kit.The data was processed using bioinformatics tools such as QIIME and the raw sequence files areavailable via NCBI's Sequence Read Archive(SRA) database.
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