UPLC PDA, MS analysis of main biologically active compounds of fruits Prunus cerasifera ehrh grown in Western Georgia.
2023
The goal of the research was to study the main bioactive compounds of wild-growing forms and fruits of some varieties of tkemali (Lat. Prunus cerasifera Ehrh) grown in Georgia, using the UPLC PDA, MS methods. In all the samples studied by us, the total amount of organic acids is quite high and ranges from 3.2% to 5.5%. In all cases, the dominant acid is malic acid (1.89% to 2.59%). More than 1/5 part of the content of citric acids falls on quinic acid (from 0.8% to 1.06%). Citric acid is the smallest in quantity in the fruit of tkemali (from 0.01% to 0.06%). As a rule, the predominant carbohydrate in tkemali fruits is up to 6.7%, its content is almost two times higher than fructose (up to 2.5%) and the indicator of sucrose is different for different varieties and forms. A high content of sucrose (more than 6.0%) is characteristic of red-leaved red-fruited varieties, regardless of the place of sampling. There have been identified 6 anthocyanin glycosides. Using the UPLC-PDA-MS method, the dominant compounds in all cases are cyanidin derivatives (cyanidin-3-0-rutinoside), and their content in the pulp, juice and skin is 12.48–9.6–25.6mg 100g -1 , respectively. Anthocyanins in the wild forms of tkemali are much higher (20.07±0.602 mg100g -1 fw), especially in the skin (112.89±3.612mg 100g -1 fw), than in cultivated varieties (4.57±0.137mg 100g -1 fw). The high antioxidant activity of tkemali fruit has been established by DPPH method.
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