Hepatitis E virus seroprevalence of domestic pigs in Germany determined by a novel in-house and two reference ELISAs
Dremsek, Paul | Joel, Sebastian | Baechlein, Christine | Pavio, Nicole | Schielke, Anika | Ziller, Mario | Duerrwald, Ralf | Renner, Christiane | Groschup, Martin H. | Johne, Reimar | Krumbholz, Andi | Ulrich, Rainer, G. | Friedrich-Loeffler-Institut (FLI) | Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany] | University of Veterinary Medicine [Hannover] | Virologie UMR1161 (VIRO) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Fed Inst Risk Assessment ; Partenaires INRAE | IDT Biol GmbH ; Partenaires INRAE | Minist Landlichen Raum Ernahrung & Verbrauchersch ; Partenaires INRAE | German Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) through the Federal Institute for Agriculture and Nutrition (BLE) [07HS026, 07HS027]; Grimminger-Stiftung
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Показать больше [+] Меньше [-]Английский. Autochthonous hepatitis E virus (HEV) infections by zoonotic transmission of genotype 3 (GT3) have been reported increasingly from industrialized countries. In this paper the development and validation of an IgG ELISA for the detection of HEV-specific antibodies in domestic pigs is described. Comparison of the diagnostic value ofEscherichia coli-expressed HEV-GT3 capsid protein (CP) derivatives revealed a carboxyterminal derivative as most suitable. Validation of the in-house assay using a commercially available IgG ELISA revealed a high diagnostic specificity and sensitivity. The average HEV seroprevalence of domestic pigs from Germany and the federal state Baden-Wuerttemberg determined by the in-house test was 42.7% and 50.3%, respectively. The seroprevalence in different districts of Baden-Wuerttemberg ranged from 34.9% to 60%, but from 0% to 100% between different herds. These data were compared to those achieved by two commercially available ELISA kits and an in-house ratHEV-based ELISA. In conclusion, the CP-based in-house test proved sensitive and specific, indicating that the ORF3-encoded protein might be dispensable for diagnostics. The novel assay also allowed a parallel analysis by a homologous ratHEV-derived antigen. Thus, the novel IgG ELISA represents a useful tool for future standardized seroprevalence studies in domestic pigs from Germany and other regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.
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