Staphylococcus aureus proteins differentially produced in ewe gangrenous mastitis or ewe milk
Le Maréchal, Caroline | Jardin, Julien | Briard-Bion, Valérie | Rault, Lucie | Berkova, Nadia | Vautor, Eric | Thiéry, Richard | Even, Sergine | Le Loir, Yves | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Unité Hygiène et Qualité des Produits Avicoles et Porcins ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Unité pathologie des ruminants ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Laboratoire vétérinaire départemental des Alpes Maritimes | Unité de pathologie des ruminants ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | INRA ANSES IMISa Project
Despite being one of the main pathogens involved in ruminant mastitis, little is known about what proteins Staphylococcus aureus does express, in vivo, during the infection. Here, two S. aureus strains were isolated from curds formed within the udder of two ewes suffering from gangrenous mastitis. Protein samples were prepared from cell fractions and were analyzed using 1D-LC MS/MS. Results were compared to 1D-LC MS/MS analysis of the same S. aureus strains grown in ewe milk. A total of 365 proteins were identified. Most of them were related to cellular metabolism, cellular division and stress response. Half of the proteins were found in both conditions but a substantial number were specifically found in in vivo conditions and gave indications about the active metabolic status and the stresses encountered by S. aureus within the cistern during a gangrenous mastitis.
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