Molecular characterisation of epizootic haemorrhagic disease virus associated with a tunisian outbreak among cattle in 2006
Ben Dhaou, Sameh | Sailleau, Corinne | Babay, Besma | Viarouge, Cyril | Sghaier, Soufien | Zientara, Stéphan | Hammami, Salah | Bréard, Emmanuel | Faculté des Sciences de Bizerte [Université de Carthage] ; Université de Carthage (Tunisie) = University of Carthage (UCAR) | Institut de Recherche Vétérinaire de Tunisie = Veterinary Research Institute of Tunis (IRVT) | Virologie UMR1161 (VIRO) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | École Nationale de Médecine Vétérinaire de Sidi Thabet ; Ministère de l’Agriculture, des Ressources Hydrauliques et de la Pêche Maritime [Tunisie] | PHC Utique programme [24849SB]
In 2006, epizootic haemorrhagic disease (EHD) outbreaks were recorded in the Maghreb (Tunisia, Morocco and Algeria) among cattle, resulting in severe repercussions on herds (oedema of the head, necrotic lesions of the oral mucosa, hyperthermia of the teats, accompanied by anorexia and respiratory distress) and economic losses. The present study gives new information on the molecular characterisation of the EHD virus (EHDV) that had circulated in Tunisia. Genome segments 2, 3, 6, 7 and 10 of EHDV, corresponding to the VP2, VP3, VP5, VP7 and NS3/NS3A proteins, respectively, were amplified from the blood of one animal by RT-PCR and sequenced. Nucleotide sequence comparisons of these five segments with sequences available in the GenBank demonstrated that an EHDV serotype 6 (EHDV-6) had been present in Tunisia in 2006. The possible origin of this strain is discussed.
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