Non-structural carbohydrates in woody plants compared among laboratories
Quentin, Audrey G. | Pinkard, Elizabeth A. | Ryan, Michael G. | Tissue, David T. | Baggett, L. Scott | Adams, Henry D. | Maillard, Pascale | Marchand, Marc | Landhäusser, Simon M. | Lacointe, André | Gibon, Yves | Anderegg, William R.L. | Asao, Shinichi | Atkin, Owen K. | Bonhomme, Marc | Claye, Caroline | Chow, Pak S. | Clément-Vidal, Anne | Davies, Noel W. | Dickman, L. Turin | Dumbur, Rita | Ellsworth, David S. | Falk, Kristen | Galiano, Lucía | Grünzweig, José M. | Hartmann, Henrik | Hoch, Günter | Hood, Sharon | Jones, Joanna E. | Koike, Takayoshi | Kuhlmann, Iris | Lloret, Francisco | Maestro, Melchor | Mansfield, Shawn D. | Martínez-Vilalta, Jordi | Maucourt, Mickael | Mcdowell, Nathan G. | Moing, Annick | Muller, Bertrand | Nebauer, Sergio G. | Palacio, Sara | Piper, Frida | Raveh, Eran | Richter, Andreas | Rolland, Gaëlle | Rosas, Teresa | Saint Joanis, Brigitte | Sala, Anna | Smith, Renee A. | Sterck, Frank | Stinziano, Joseph R. | Tobias, Mari | Unda, Faride | Watanabe, Makoto | Way, Danielle A. | Weerasinghe, Lasantha K. | Wild, Birgit | Wiley, Erin | Woodruff, David R. | Commonwealth Scientific and Industrial Research Organisation [Australia] (CSIRO) | Western Sydney University | Colorado State University [Fort Collins] (CSU) | United States Department of Agriculture (USDA) | Los Alamos National Laboratory (LANL) | Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | University of Alberta | Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF) ; Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP) | Biologie du fruit et pathologie (BFP) ; Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB) | Plateforme Bordeaux Metabolome ; Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Bordeaux ; MetaboHUB-MetaboHUB | Princeton University | Australian National University (ANU) | University of Tasmania [Hobart] (UTAS) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | The Hebrew University of Jerusalem (HUJ) | Oregon State University (OSU) | Swiss Federal Institute for Forest, Snow and Landscape Research WSL | Albert-Ludwigs-Universität Freiburg = University of Freiburg | Max-Planck-Institut für Biogeochemie (MPI-BGC) | Université de Bâle = University of Basel = Basel Universität (Unibas) | Montana State University (MSU) | Hokkaido University [Sapporo, Japan] | Universitat de Barcelona (UB) | Instituto Pirenaico de Ecologìa = Pyrenean Institute of Ecology [Zaragoza] (IPE - CSIC) ; Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC) | University of British Columbia [Canada] (UBC) | Écophysiologie des Plantes sous Stress environnementaux (LEPSE) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Universitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia (UPV) | Centro de Investigación en Ecosistemas de la Patagonia - Universidad Austral de Chile (CIEP) | Universität Wien = University of Vienna | Wageningen University and Research [Wageningen] (WUR) | University of Western Ontario (UWO) | Estonian University of Life Sciences (EMU) | Tokyo University of Agriculture and Technology (TUAT) | Duke University [Durham] | University of Peradeniya | Göteborgs Universitet = University of Gothenburg (GU) | USDA Agricultural Research Service [Maricopa, AZ] (USDA) ; United States Department of Agriculture (USDA)
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Показать больше [+] Меньше [-]Английский. Non-structural carbohydrates (NSC) in plant tissue are frequently quantified to make inferences about plant responses to environmental conditions. Laboratories publishing estimates of NSC of woody plants use many different methods to evaluate NSC. We asked whether NSC estimates in the recent literature could be quantitatively compared among studies. We also asked whether any differences among laboratories were related to the extraction and quantification methods used to determine starch and sugar concentrations. These questions were addressed by sending sub-samples collected from five woody plant tissues, which varied in NSC content and chemical composition, to 29 laboratories. Each laboratory analyzed the samples with their laboratory-specific protocols, based on recent publications, to determine concentrations of soluble sugars, starch and their sum, total NSC. Laboratory estimates differed substantially for all samples. For example, estimates for Eucalyptus globulus leaves (EGL) varied from 23 to 116 (mean = 56) mg g(-1) for soluble sugars, 6-533 (mean = 94) mg g-1 for starch and 53-649 (mean = 153) mg g-1 for total NSC. Mixed model analysis of variance showed that much of the variability among laboratories was unrelated to the categories we used for extraction and quantification methods (method category R-2 = 0.05-0.12 for soluble sugars, 0.10-0.33 for starch and 0.01-0.09 for total NSC). For EGL, the difference between the highest and lowest least squares means for categories in the mixed model analysis was 33 mg g-1 for total NSC, compared with the range of laboratory estimates of 596 mg g-1. Laboratories were reasonably consistent in their ranks of estimates among tissues for starch (r = 0.41-0.91), but less so for total NSC (r = 0.45-0.84) and soluble sugars (r = 0.11-0.83). Our results show that NSC estimates for woody plant tissues cannot be compared among laboratories. The relative changes in NSC between treatments measured within a laboratory may be comparable within and between laboratories, especially for starch. To obtain comparable NSC estimates, we suggest that users can either adopt the reference method given in this publication, or report estimates for a portion of samples using the reference method, and report estimates for a standard reference material. Researchers interested in NSC estimates should work to identify and adopt standard methods.
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