Probing the threshold of membrane damage and cytotoxicity effects induced by silica nanoparticles in Escherichia coli bacteria
2017
Mathelié-Guinlet, Marion | Béven, Laure | Moroté, Fabien | Moynet, Daniel | Grauby-Heywang, Christine | Gammoudi, Ibtissem | Delville, Marie-Hélène | Cohen-Bouhacina, Touria | Laboratoire Ondes et Matière d'Aquitaine (LOMA) ; Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) | Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) ; Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | Biologie du fruit et pathologie (BFP) ; Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB) | Biothérapies des maladies génétiques et cancers ; Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM) | Cellule de transfert ADERA NanoPhyNov | the Région Aquitaine and CNRS (France) for supporting this work through the equipment of the NanoSpectroImagerie (NSI-LOMA) platform used in The authors thank this work (CPER COLA2). They are grateful to the Direction Générale de l'Armement (DGA, Ministère de la Défense France, grant number : 2014017) and the Région Aquitaine (France) for their financial support through the Ph.D. grant of M. Mathelié-Guinlet. Finally, authors thank NSI-LOMA platform for technical help and J.P Chapel for its helpful advises and discussions about DLS measurements.
UMR 1332 - Equipe Mollicutes
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Показать больше [+] Меньше [-]Английский. The engineering of nanomaterials, because of their specific properties, is increasingly being developed for commercial purposes over the past decades, to enhance diagnosis, cosmetics properties as well as sensing efficiency. However, the understanding of their fate and thus their interactions at the cellular level with bio-organisms remains elusive. Here, we investigate the size-and charge-dependence of the damages induced by silica nanoparticles (SiO 2-NPs) on Gram-negative Escherichia coli bacteria. We show and quantify the existence of a NPs size threshold discriminating toxic and inert SiO 2-NPs with a critical particle diameter (Φ c) in the range 50nm–80nm. This particular threshold is identified at both the micrometer scale via viability tests through Colony Forming Units (CFU) counting, and the nanometer scale via atomic force microscopy (AFM). At this nanometer scale, AFM emphasizes the interaction between the cell membrane and SiO 2-NPs from both topographic and mechanical points of view. For SiO 2-NPs with Φ > Φ c no change in E. coli morphology nor its outer membrane (OM) organization is observed unless the NPs are positively charged in which case reorganization and disruption of the OM are detected. Conversely, when Φ < Φ c , E. coli exhibit unusual spherical shapes, partial collapse, even lysis, and OM reorganization.
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