A two-year monitoring of pesticide hazard in-hive: High honey bee mortality rates during insecticide poisoning episodes in apiaries located near agricultural settings

Pesticide residues in beebread, live and dead honey bees, together with honey bee death rate were monitored from June 2016 to June 2018 in three apiaries, located near agricultural settings and in wildlands. Dead honey bees were only collected and analyzed when significant mortality episodes occurred and pesticide content in beeswax of each experimental apiary was evaluated at the beginning of the study. Samples were extracted by a modified QuEChERS procedure and screened for pesticides residues by liquid chromatography mass spectrometry (LC-MS/MS). Pesticide hazard in the samples was evaluated through the hazard quotient approach (HQ). Beebread was widely contaminated with coumaphos and amitraz degradate 2, 4-dimethylphenylformamide (DMF), miticides detected in 94 and 97% of samples respectively. However, insecticides sprayed during citrus bloom like chlorpyrifos (up to 167 ng g )and dimethoate (up to 34 ng g )were the main responsible of the relevant pesticide hazard in this matrix. Pesticide levels in live bees were mostly residual, and pesticide hazard was low. Beeswax of the apiaries, contaminated by miticides, revealed a low pesticide hazard to honey bee colonies. Acute mortality episodes occurred only in the two apiaries located near agricultural settings. Dead bees collected during these episodes revealed high levels (up to 2700 ng g )of chlorpyrifos, dimethoate, omethoate and imidacloprid. HQ calculated in dead bees exceeded up to 37 times the threshold value considered as elevated hazard to honey bee health.

This work has been suported by the Spanish Ministerio de Agricultura, Alimentación y Medio Ambiente through the project 20160020000834: “Análisis de la Pérdida de Viabilidad y Despoblamiento de las Colonias de Abejas (Apis mellifera L.) Mediante el Mapeo de Xenobióticos” and through the project ANTROPOCEN@ (PROMETEO/2018/155) by Generalitat Valenciana. Pau Calatayud-Vernich acknowledges the Foundation “Tatiana Pérez de Guzmán el Bueno” for the financial support through PhD Environmental Fellowship Program. We thank the Agrupación de Defensa Sanitaria Apícola (apiADS) for their help with counseling and sampling