Exploiting host immunity and parasite genomics to develop a robust sub-unit vaccine against East Coast fever in cattle - Where are we?
Mwangi, David Miano | Graham, S.P. | Pelle, Roger | Honda, Y. | Tonukari, N.J. | Yamage, I.M. | Glew, E.J. | Villiers, Etienne P. de | Shah, Tushaar | Bishop, Richard P. | Abuya, E. | Awino, Elias | Gachanja, J. | Luyai, A.E. | Mbwika, F. | Muthiani, A.M. | Ndegwa, D.M. | Njahira, Moses N. | Nyanjui, J.K. | Onono, F.O. | Osaso, J. | Saya, Rosemary M. | Wildmann, C. | Fraser, C.M. | Maudlin, I. | Gardner, M.J. | Morzaria, S.P. | Loosmore, S. | Gilbert, S.C. | Audonnet, J.C. | Bruggen, P. van der | Nene, Vishvanath M. | Taracha, E.L.N.
Theileria parva, a tick-borne api-complexan protozoan parasite, causes East Coast fever (ECF) in cattle. Control of the disease by improved vaccination is believed to provide a sustainable solution. Class I MHC-restricted CD8+ cytotoxic T lymphocytes (CTL) directed at schizontinfected cells constitute the effector immune mechanism against ECF in cattle recovering from a single or multiple infection(s). Schizont antigens recognised by CTL are therefore prime vaccine candidates. We describe a rational approach used to identify 8 CTL target antigens as vaccine candidates. The genes encoding the target antigens have been engineered in plasmid DNA and viral vectors for evaluating their immunogenicity and efficacy in cattle. In a preliminary trial, five of the candidate vaccines demonstrated the capacity to induce CTL responses that correlated with survival and reduced disease severity following a lethal parasite challenge.
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Эту запись предоставил International Livestock Research Institute