Individual whole-animal parameters of Polar cod (Boreogadus saida) and Atlantic cod (Gadus morhua) acclimated to ocean acidification and warming conditions
2016
Kunz, Kristina Lore | Frickenhaus, Stephan | Hardenberg, Silvia | Torild, Johansen | Leo, Elettra | Pörtner, Hans-Otto | Schmidt, Matthias | Windisch, Heidrun Sigrid | Knust, Rainer | Mark, Felix Christopher
In order to access potential impacts of ocean acidification and warming on the whole-animal performance of polar cod (Boreogadus saida) and Atlantic cod (Gadus morhua), individuals of both species were acclimated to different temperature (polar cod: 0, 3, 6, 8°C; Atlantic cod: 3, 8, 12, 16°C) and PCO2 (390 and 1,170 µatm) scenarios for four months. Wet body weight (BW) (g) and total length (TL) (mm) of each individual were measured before and after the incubation period. Subsequently, the specific growth rate (SGR) (%/day) was calculated. The fish were fed every fourth day. Individual feed consumption (FI) (g) was recorded and used for the calculation of daily feed intake (g/(day*BW)). Growth gain (g) and FI throughout the incubation period were translated into feed conversion efficiency (FCE). After the incubation period, starved fish were placed in a respiration chamber to identify the standard metabolic rate (SMR) (µmol/(min*g)) using intermittent-flow respirometry. Subsequently, the fish were sacrificed for the determination of BW, TL, liver weight (WL) (g), gonad weight (WG) (g), stomach including content (g) and stomach content (g) for the calculation of condition factor (CF), hepatosomatic index (HSI) (%), gonadosomatic index (GSI) (%) and stomach filling (%).
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