Ultrasound-Assisted Extraction of Total Phenolic Compounds and Antioxidant Activity in Mushrooms
Aliaño González, María José | Barea Sepúlveda, Marta | Espada Bellido, Estrella | Ferreiro González, Marta | López-Castillo, José Gerardo | Palma Lovillo, Miguel | Fernández Barbero, Gerardo | Carrera Fernández, Ceferino Adrián | Química Analítica
The consumption of mushrooms has considerably increased in recent years because of their beneficial nutritional properties due to their essential amino acids, proteins, and dietary fiber content. Recent research has shown that they are also rich in polysaccharides and phenolic compounds. These compounds exhibit decisive free radical and ROS scavenging power with potential application to the treatment of neurodegenerative disorders. In addition, they present important properties like antioxidant, antiaging, and immune modulation. In the present research, the optimization for the extraction of total phenolic compounds and the antioxidant activity (DPPH and ABTS), based on ultrasound-assisted techniques has been carried out. Five variables (% MeOH in solvent, extraction temperature, amplitude, cycle, and sample:solvent ratio have been selected; both the total phenolic compounds content as well as the antioxidant activity (DPPH and ABTS)) have been considered as the response variables. The optimal conditions, determined by means of a multiresponse optimization method, were established at 0.2 g of sample extracted with 15.3 mL of solvent (93.6% MeOH) at 60 degrees C for 5 min and using 16.86% amplitude and 0.71 s(-1) cycles. A precision study of the optimized method has been performed with deviations lower than 5%, which proves the repeatability and precision of the extraction method. Finally, the extraction method has been applied to wild and commercial mushrooms from Andalusia and Northern Morocco, which has confirmed its suitability for the extraction of the phenolic compounds from mushroom samples, while ensuring maximum antioxidant activity.
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