Genome wide association mapping of end-use gluten properties in bread wheat landraces (Triticum aestivum L.)
López Fernández, Matilde | Chozas Hernández, Alejandro | Benavente Barzana, Maria Elena | Alonso Rueda, Elia | Isidro Sánchez, Julio | Pascual Bañuls, Laura | Giraldo Carbajo, Patricia
Numerous studies have aimed to dissect the genetic basis of wheat end-use quality, yet the consideration of gluten aggregation properties in this context has been limited despite its important role in determining the processing quality. This study has addressed the evaluation of the end-use quality of a collection of 189 Spanish bread wheat (Triticum aestivum L.) landraces. For phenotyping, we utilized the GlutoPeak methodology to assess gluten aggregation traits, the SDS-sedimentation test to measure gluten strength and NIR for grain protein content. In addition to previous genotyping data with numerous DArT-seq markers distributed across the entire wheat genome, we developed ten KASP-based molecular markers designed for GLU-1 loci, which encode high molecular weight glutenins (HMW-GS). The reliability of these markers was thoroughly assessed. Our genomewide association analysis (GWAS) focused on gluten properties and protein content, revealed 89 marker-traitassociated loci (MTAs). These loci were further grouped into 50 MTA-QTLs across various chromosomes based on linkage disequilibrium. While some MTA-QTLs overlapped with regions identified in other studies, others represented novel genomic regions. Inside these regions, we identified several candidate genes with gluten quality-related annotated functions or grain-specific expression patterns, which represent potential targets for marker development and future wheat end-use quality improvement.
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Эту запись предоставил Universidad Politécnica de Madrid