Meta-topolin-mediated regeneration and accumulation of phenolic acids in the critically endangered medicinal plant Crinum malabaricum (Amaryllidaceae): A potent source of galanthamine
Chahal, Swati | Kaur, Harmeet | Lekhak, Manoj | Shekhawat, Mahipal | Goutam, Umesh | Singh, Sachin Kumar | Ochatt, Sergio | Kumar, Vijay | Lovely Professional University, Punjab, India | Shivaji University, Vidyanagar, Kolhapur, Maharashtra, India | Department of Botany, Kanchi Mamuniver Government Institute for Postgraduate Studies and Research | Agroécologie [Dijon] ; Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | SC thank the DST-SERB, New Delhi, India for support in the formof fellowship. We also acknowledge SERB, DST, Govt. of India (FileNo. SRG/2019/001279) for the financial support to conduct thisstudy.
International audience
Показать больше [+] Меньше [-]Английский. Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant endemic to India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the use of meta-Topolin (mT) on in vitro regeneration of C. malabaricum. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5, 2.5, 5.0, 7.5 and 10.0 µM mT for six weeks, whereby 7.5 µM mT resulted in the maximum multiplication of adventitious shoots, much higher than the control. The biochemical accumulation of eleven different phenolic acids was quantified by UHPLC-MS/MS analysis, and it appeared that mT-treated cultures exhibited the highest concentration of phenolic acids. In particular, increased concentrations of gallic acid, protocatechuic acid, syringic acid, p-hydroxybenzoic acid, salicylic acid and vanillic acid were detected compared to the control. mT (2.5 and 5.0 µM) produced the maximum amount of chlorogenic acid, ferulic acid, p-coumaric acid and sinapic acid. However, an increased content of caffeic acid was produced on PGR-free medium. These findings highlight the beneficial effect and validate the rising importance of mT for in vitro regeneration studies. This study will serve as a potential protocol to conserve and restore the medically important C. malabaricum.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Institut national de la recherche agronomique