Aurora-C interacts with and phosphorylates the transforming acidic coiled-coil 1 protein.
2011
Gabillard, Jean-Charles | Ulisse, Salvatore | Baldini, Enke | Sorrenti, Salvatore | Cremet, Jean-Yves | Coccaro, Carmela | Prigent, Claude | d'Armiento, Massimino | Arlot-Bonnemains, Yannick | Institut de Génétique et Développement de Rennes (IGDR) ; Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) | Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) ; Institut National de la Recherche Agronomique (INRA) | Department of Experimental Medicine ; Università degli Studi di Roma "La Sapienza" = Sapienza University [Rome] (UNIROMA) | Department of Surgical Sciences ; Università degli Studi di Roma "La Sapienza" = Sapienza University [Rome] (UNIROMA)
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Показать больше [+] Меньше [-]Английский. Aurora-C, a member of the Aurora kinase family, is implicated in the regulation of mitosis. In contrast to Aurora-A and Aurora-B its cellular localization and functions are poorly characterized. TACC1 protein belongs to the transforming acidic coiled-coil family shown to interact with the Aurora kinases. In the present study we analyzed the interaction between Aurora-C and TACC1 by means of immunofluorescence (IF), co-immunoprecipitation (IP) and in vitro phosphorylation experiments. We demonstrated that Aurora-C and TACC1 proteins co-localize to the midbody of HeLa cells during cytokinesis. Immunoprecipitated TACC1 from HeLa cell extracts was associated with Aurora-C. In addition, the interaction of the two proteins was tested by analyzing the phosphorylation of TACC1 in vitro. The results demonstrated that TACC1 is phosphorylated by Aurora-C on a serine at position 228. In conclusion, the study demonstrated that TACC1 localizes at the midbody during cytokinesis and interacts with and is a substrate of Aurora-C, which warrant further investigation in order to elucidate the functional significance of this interaction.
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