Comparative proteomics on deep-sea amphipods after in situ copper exposure

The interest in deep-sea mining increased along with the environmental concerns of these activities to the deep-sea fauna. The discovery of optimal biomarkers of deep-sea mining activities in deep-sea species is a crucial step toward the supply of important ecological information for environmental impact assessment. In this study, an in situ copper exposure experiment was performed on deep-sea scavenging amphipods. Abyssorchomene distinctus individuals were selected among all the exposed amphipods for molecular characterization. Copper concentration within the gut was assessed, followed by a tandem mass tag-based coupled with two-dimensional liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) applied to identify and quantify the protein expression changes after 48 h of exposure. 2937 proteins were identified and annotated, and 1918 proteins among all identified proteins were assigned by at least two nonambiguous peptides. The screening process was performed based on the differences in protein abundance and the specific correlation between the proteins and copper in previous studies. These differentially produced proteins include Na+/K+ ATPase, cuticle, chitinase, and proteins with unknown function. Their abundances showed correlation with copper and had high sensitivity to indicate the copper level, being here proposed as biomarker candidates for deep-sea mining activities in the future. This is a key step in the development of environmental impact assessment of deep-sea mining activities integrating ecotoxicological data.

Funding Agency National Key Research Program of China 2016YFC0304105 China Ocean Mineral Resources R&D Association Program DY135-E2-1-03 Hong Kong Branch of South Marine Science and Engineering Guangdong Laboratory Fundacao para a Ciancia e Tecnologia I.P. Portugal (FCT) Direcao-Geral de Politica do Mar (DGPM) through the project Mining-MiningImpact 2 (JPI Oceans) 2/2017/001 Portuguese Foundation for Science and Technology CEECIND005262017

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