The Bacteriophage Pf-10—A Component of the Biopesticide “Multiphage” Used to Control Agricultural Crop Diseases Caused by <i>Pseudomonas syringae</i>
Olesya A. Kazantseva | Rustam M. Buzikov | Tatsiana A. Pilipchuk | Leonid N. Valentovich | Andrey N. Kazantsev | Emilia I. Kalamiyets | Andrey M. Shadrin
Phytopathogenic pseudomonads are widespread in the world and cause a wide range of plant diseases. In this work, we describe the <i>Pseudomonas</i> phage Pf-10, which is a part of the biopesticide “Multiphage” used for bacterial diseases of agricultural crops caused by <i>Pseudomonas syringae</i>. The Pf-10 chromosome is a dsDNA molecule with two direct terminal repeats (DTRs). The phage genomic DNA is 39,424 bp long with a GC-content of 56.5%. The Pf-10 phage uses a packaging mechanism based on T7-like short DTRs, and the length of each terminal repeat is 257 bp. Electron microscopic analysis has shown that phage Pf-10 has the podovirus morphotype. Phage Pf-10 is highly stable at pH values from 5 to 10 and temperatures from 4 to 60 °C and has a lytic activity against <i>Pseudomonas</i> strains. Phage Pf-10 is characterized by fast adsorption rate (80% of virions attach to the host cells in 10 min), but has a relatively small number of progeny (37 ± 8.5 phage particles per infected cell). According to the phylogenetic analysis, phage Pf-10 can be classified as a new phage species belonging to the genus <i>Pifdecavirus</i>, subfamily <i>Studiervirinae</i>, family <i>Autographiviridae</i>, order <i>Caudovirales</i>.
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