Sodium houttuyfonate effectively treats acute pulmonary infection of Pseudomonas aeruginosa by affecting immunity and intestinal flora in mice
2022
Tian Zhuang | Tian Zhuang | Mengxue Hu | Mengxue Hu | Jian Wang | Jian Wang | Longfei Mei | Longfei Mei | Xiaoxiao Zhu | Xiaoxiao Zhu | Haitao Zhang | Haitao Zhang | Feng Jin | Feng Jin | Jing Shao | Jing Shao | Jing Shao | Tianming Wang | Changzhong Wang | Changzhong Wang | Changzhong Wang | Xiaojia Niu | Xiaojia Niu | Xiaojia Niu | Daqiang Wu | Daqiang Wu
IntroductionPseudomonas aeruginosa is a major nosocomial pathogen that frequently causes ventilator-associated pneumonia in specific populations. Sodium houttuyfonate (SH) has shown mild antibacterial activity against P. aeruginosa in vitro, but the mechanism of potent antimicrobial activity of SH against P. aeruginosa infection in vivo remains unclear.MethodsHere, using the mouse pneumonia model induced by P. aeruginosa nasal drip to explore the therapeutic effects of SH.ResultsWe found that SH exhibits dose-dependent therapeutic effects of reducing P. aeruginosa burden and systemic inflammation in pneumonia mice. SH ameliorates inflammatory gene expression and production of inflammatory proteins, such as interleukin-6 (IL-6), nuclear factor kappa-B (NF-κB) and toll-like receptor 4 (TLR4), associated with the TLR4/NF-κB pathway in mice with P. aeruginosa pneumonia. Furthermore, we analyzed the intestinal flora of mice and found that compared with the model group, the abundance and diversity of beneficial bacterial flora of SH treatment groups increased significantly, suggesting that SH can improve the intestinal flora disorder caused by inflammation. In addition, SH improves alpha and beta diversity index and reduces species abundance differences of intestinal flora in pneumonia mice.DiscussionTaken together, our presented results indicate that SH may effectively alleviate the acute pulmonary infection induced by P. aeruginosa by reducing the disturbance of regulating immunity and intestinal flora in mice.
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