Isolation and Characterization of a Lytic <i>Vibrio parahaemolyticus</i> Phage vB_VpaP_GHSM17 from Sewage Samples
Xunru Liang | Yuhang Wang | Bin Hong | Yanmei Li | Yi Ma | Jufang Wang
<i>Vibrio parahaemolyticus</i> is a major foodborne pathogen and the main cause of diarrheal diseases transmitted by seafood such as fish, shrimp, and shellfish. In the current study, a novel lytic phage infecting <i>V. parahaemolyticus</i>, vB_VpaP_GHSM17, was isolated from the sewage of a seafood market, Huangsha, Guangzhou, and its morphology, biochemistry, and taxonomy features were identified. Morphological observation revealed that GHSM17 had an icosahedral head with a short, non-contractile tail. The double-stranded DNA genome of GHSM17 consisted of 43,228 bp with a GC content of 49.42%. In total, 45 putative ORFs were identified in the GHSM17 genome. Taxonomic analysis indicated GHSM17 belonging to genus <i>Maculvirus</i>, family <i>Autographiviridae.</i> In addition, GHSM17 was stable over a wide range of temperatures (20–60 °C) and pH (5–11) and was completely inactivated after 70 min of ultraviolet irradiation. The bacterial inhibition assay revealed that GHSM17 could inhibit the growth of <i>V. parahaemolyticus</i> within 8 h. The results support that phage GHSM17 may be a potential candidate in the biological control of <i>V. parahaemolyticus</i> contamination in aquaculture.
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