Synthesis of cocoa butter triacylglycerols using a model acidolysis system
Ozan Nazim Çiftçi | Sibel Fadiloǧlu | Boleslaw Kowalski | Fahrettin Göǧüş
The effects of reaction parameters such as substrate mole ratio, reaction temperature, enzyme load, water content and reaction time were studied in a model enzymatic acidolysis system. Palmitic and stearic acids were incorporated into triolein (OOO) under the catalysis of sn-1,3 specific lipase to produce the three major triacylglycerols (TAGs) in cocoa butter (CB), namely, 1,3-dipalmitoyl-2-oleoyl--glycerol (POP), 1(3)-palmitoyl-3(1)-stearoyl-2-oleoyl-glycerol (POS) and 1,3distearoyl-2-oleoyl-glycerol (SOS). TAG contents of the reaction products were analyzed by High Performance Liquid Chromatograph (HPLC).The best results (15.2% POP, 30.4% POS, 15.2 % SOS) were obtained at 1:3:3 (OOO:palmitic acid:stearic acid) substrate mole ratio and reaction parameters: time 10 h, temperature 45 oC, enzyme load 20 %, water content 5 %. The results obtained in this model system might be used for the optimization and application of lipase catalyzed acidolysis reactions in natural systems to produce cocoa butter equivalents (CBEs).
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Directory of Open Access Journals