Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases
Şükrü Önalan | Aslı ÇİLİNGİR YELTEKİN
In this study, symptomatic fish samples were taken from rainbow trout farms. Isolation and identification of agents isolated from fish samples were made. DNA isolations from different purified colonies were carried out with the mericon bacterial DNA kit. Real-Time PCR procedure was performed by using universal bacterial primers. Molecular identifications were performed by blasting the nucleotides obtained by sequence analysis of PCR amplicons. Spectrophotometric measurements were performed at 412 nm wavelengths for AChE activity and 412 nm for BChE activity from liver and brain tissues of fish samples. The activity differences of different disease factors among themselves and according to the control group were examined. As a result of the study, isolation and identification of Bacillus subtilis, Lactococcus garvieae and Staphylococcus epidermidis from 5 different farms were performed. Over 98% similarity was observed as a result of sequencing analysis of the isolates. In this study, it was observed that three different bacteria isolated from trout farms suppressed AChE and BChE enzyme activities in both tissues of trout.
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