Anther culture studies in W. Murcott mandarin genotype
Yıldız AKA KAÇAR | Özhan ŞİMŞEK | Dicle DÖNMEZ | Melda BONCUK | Turgut YEŞİLOĞLU | Mehmet AKGÖL
Obtaining haploid plants is one of the important tool that shorten the plant breeding process. Anther culture is one of the method used for obtaining haploid plants. Anther culture studies are carried out for several plant species. In the present study, flowers at different sizes of W. Murcott mandarin were collected at the blooming period. Stage of mononuclear microspore of anthers was determined according to method of acetocarmine. Mononuclear microspore stage of anthers was observed in flowers 4-5 mm in diameter. Anther culture experiments were carried out with 4-5 mm in diameter anthers. In this study, temperature pre-treatment was performed at 4˚C and 25˚C for two days. Surface sterilization was performed in flowers and anthers were cultured in nutrient medium N6. Anther culture assays were performed with the three different concentrations of TDZ (0 mg l-1 TDZ, 0.1 mg l-1 TDZ, 0.5 mg l-1 TDZ). Half of anthers were cultured in dark condition while the other half in 16 hours light, 8 hours dark climate room conditions. Anthers containing mononuclear microspore stage were cultured and, changes occurring in the anther explants were discussed in the article.
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