Determination of sharka virus disease in Antalya
Nejla ÇELİK | Bengi TOPKAYA KÜTÜK
This study was conducted in stone fruit trees in Antalya region in 2008-2011. In this respect, a survey was accomplished in stone fruit trees (apricot, plum, peach, cherry and nectarine) cultivated in Antalya city center, Aksu, Serik, Alanya, Manavgat, Gazipaşa, Korkuteli, Elmalı, Finike and Kaş districts to evaluate the situation of Plum pox potyvirus, the agent of sharka disease. Samplings were conducted in both orchards and individual stone fruit trees. In the study, a total of 426 leaf samples were tested by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) serologic method. As a result of serologic tests, samples of nectarine trees obtained from a garden were only positive and these samples were sent to Ankara Plant Protection Central Research institute’s virology laboratory for verification by polymerase chain reaction (PCR). Positive samples by PCR tests were considered as PPV-infected.
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