Callus induction and biomass accumulation in vitro in explants from chokeberry (Aronia melanocarpa (Michx.) Elliot) fruit
Tatiana I. Calalb | Anatolie C. Nistreanu | Silvia G. Oroian | Mihaela M. Samarghitan
In this study, the following features were determined: biological (the optimal histogen as explant and the optimal age of donor fruit), biotechnological (type, dosage and combination of growth regulators supplements in culture medium Murashige and Skoog as well as sucrose dosage), and physical (light regime), to induce callusing and biomass accumulation in vitro from the succulent chokeberry (Aronia melanocarpa (Michx.) Elliot) fruit. It turned out that it was much easier to induce callus from explants composed of the epicarp and hypoderm cut from fruits at 50–60 days after flowering. The role of light regime and varied supplementation of the basic MS medium with different doses of growth regulators was established; they resulted in four pigmented carpomass: violet, cream-pink, cream-white and green. The best combinations for the proliferation of fruit callus were culture media with 0.2–2.5 mg × dm-3 2,4-D+0.5 mg × dm-3 KIN +60 g × dm-3sucrose, while for fruit biomass accumulation enriched with phenolic substances – 2.5–3.5 mg × dm-3 NAA+0.5 mg × dm-3 KIN+60 g × dm-3sucrose. The chemical study of phenolic compounds by HPLC coupled with the mass spectrometry method identified chlorogenic acid, hiperozide, quercetrin, isoquercitrin and rutozide quantitatively and qualitatively in all pigmented carpomass and fruits; an exception is p-coumaric present only qualitatively in green carpomass and absent in fruit and quercetol absent in green carpomass.
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