Effect of the way of cutting and dividing shoots on the <em>in vitro</em> multiplication of three cultivars of bananas and plantains
Silvio Martínez | M. Fontes-Leandro | O. Pérez-Espinosa | M. Aday-Reyez | Leyanys García- Águila | Y. Rodríguez Cruz | A. Valdés- Moreno
Propagation of bananas and plantains (Musa spp.) through biotechnological techniques has generated a great demand for biofactories. However, it is necessary to optimize the production process to make it more efficient. This work was carried out to determine the effect of the way of cutting and dividing shoots on the in vitro multiplication of three cultivars of bananas and plantains (‘Grande naine’, ‘FHIA 18’ and ‘FHIA 21’). Side shoots (1.0 cm height and 0.5 cm diameter of pseudostem) were used as plant material. Two subcultures on medium containing MS salts, 4.0 gl-1 agar, 0.65 mgl-1 IAA, 30 gl- 1 sucrose, and 4.0, 4.0, 3.0 mgl-1 6-BAP for the cultivars ‘Grande naine’, ‘FHIA-18’ and ‘FHIA-21’, were carried out respectively. Seven forms of cutting and dividing shoots were evaluated. A significant increase in the number of shoots per explant, in all cultivars tested, was reached when a longitudinal section of micro-corm up to the apical dome and without dividing pseudostem was practiced. Additionally, plants required to be transferred to the rooting phase in liquid culture media were obtained by this procedure. Key words: apical dome, ‘FHIA-18’, ‘FHIA-21’, ‘Grande naine’, micro-corm, pseudostem
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