Association Study of Symbiotic Genes in Pea (<i>Pisum sativum</i> L.) Cultivars Grown in Symbiotic Conditions
Vladimir A. Zhukov | Aleksandr I. Zhernakov | Anton S. Sulima | Olga A. Kulaeva | Marina S. Kliukova | Alexey M. Afonin | Oksana Y. Shtark | Igor A. Tikhonovich
In garden pea (<i>Pisum sativum</i> L.), several symbiotic genes are known to control the development of mutualistic symbioses with nodule bacteria (NB) and arbuscular mycorrhizal fungi (AMF). Here, we studied whether the allelic state of the symbiotic genes was associated with the growth parameters of pea plants under single inoculation with NB and under double inoculation with NB + AMF. Using different statistical methods, we analyzed the dataset obtained from a pot experiment that involved 99 pea cultivars, 10 of which were characterized as having shortened internodes due to the presence of the natural mutation p.A229T in the developmental gene <i>Le</i>. The plant’s habitus strongly influenced most of the studied growth and yield parameters and the effectiveness of the symbiotic interactions under NB and NB + AMF inoculation. Double inoculation had different effects on <i>Le</i><sup>+</sup> (normal) and <i>le</i><sup>−</sup> (dwarf) plants with regard to nitrogen and phosphorus content in seeds. Regardless of the <i>Le</i>-status of plants, allelic states of the symbiotic gene <i>LykX</i> encoding the putative receptor of Nod factors (bacterial signal molecules) were shown to be associated with seed number, thousand-seed weight, and pod number at the level of FDR < 0.001, whereas associations of allelic states of the other studied symbiotic genes were less significant.
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